Plaetje:Summary of SAGE.svg
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Besjrieving
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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
| Datum | |
| Brón | Eige werk |
| Sjriever | Thomas Shafee |
| Anger versies |
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- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
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| Datum/Tied | Miniatuurplaetje | Aafmaetinge | Gebroeker | Kómmentaar | |
|---|---|---|---|---|---|
| hujig | 13 fib 2017 14:21 | | 938 × 756 (180 kB) | Evolution and evolvability | text as paths to avoid artefacts |
| 13 fib 2017 12:48 |  | 938 × 756 (104 kB) | Evolution and evolvability | update sequence text to 'sans-serif' open font for more robust rendering | |
| 7 okt 2016 07:27 |  | 938 × 756 (104 kB) | Evolution and evolvability | ce | |
| 7 okt 2016 07:23 |  | 938 × 756 (102 kB) | Evolution and evolvability | ce | |
| 7 okt 2016 07:16 |  | 938 × 756 (104 kB) | Evolution and evolvability | digitag-->tag | |
| 5 okt 2016 07:08 |  | 1.020 × 821 (106 kB) | Evolution and evolvability | User created page with UploadWizard |
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